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Title
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Porcine reproductive and respiratory syndrome virus modulates apoptosis during replication in alveolar macrophages
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Author
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Abstract
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| Different viruses have evolved strategies that inhibit apoptosis of the host cell early in infection and/or induce apoptosis in the host cell late in infection. In this study, it was investigated if and when porcine reproductive and respiratory syndrome virus (PRRSV) modulates apoptosis in PRRSV-infected macrophages. The PRRSV replication cycle in macrophages was completed within 12 h post-inoculation (hpi). PRRSV-infected macrophages, treated with staurosporine at 4, 5, 6 and 8 hpi, were significantly protected against staurosporine-induced apoptosis, but PRRSV-infected macrophages, treated with staurosporine at 12 hpi, were not. In contrast, starting from 12 hpi, all PRRSV-infected macrophages died by caspase-dependent apoptosis, which culminated in secondary necrosis. Treatment of PRRSV-infected macrophages with Z-Val-DL-Asp-fluoromethylketone indicated that apoptosis late in infection was not essential for efficient virus release. Anti- and pro-apoptotic activities were also observed in PRRSV-infected Marc-145 cells. In conclusion, this study shows that PRRSV stimulates anti-apoptotic pathways in macrophages early in infection and that PRRSV-infected macrophages die by apoptosis late in infection. |
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Language
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English
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Source (journal)
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Archives of virology. - Wien, 1975, currens
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Publication
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Wien
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2008
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ISSN
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0304-8608
[print]
1432-8798
[online]
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DOI
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10.1007/S00705-008-0135-5
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Volume/pages
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153
:8
(2008)
, p. 1453-1465
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ISI
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000257713600006
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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