Title
Gene expression profiling of porcine alveolar macrophages after antibody-mediated cross-linking of sialoadhesin (Sn, Siglec-1) Gene expression profiling of porcine alveolar macrophages after antibody-mediated cross-linking of sialoadhesin (Sn, Siglec-1)
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
New York ,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Journal of receptor and signal transduction research. - New York
Volume/pages
28(2008) :3 , p. 185-243
ISSN
1079-9893
ISI
000256839700004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Sialoadhesin (Sn) is the prototypic member of the Siglecs, a family of receptors mainly involved in cell-cell interactions. For several Siglecs, but not for Sn, intracellular signaling functions have been described. Because antibody-mediated cross-linking of surface transmembrane proteins is a powerful technique to investigate cell-molecular events, Sn expressed on porcine alveolar macrophages (PAM) was cross-linked with the antibody 41D3, and the expression profiles were compared with mock-treated macrophages by microarray analysis. Gene ontology analysis of 479 differentially expressed transcripts identified gene categories related to membrane localization, signal transduction, receptor and communication activities. Analyses of the human KEGG pathway database identified MAP kinase signaling, regulation of actin cytoskeleton, adipocytokine signaling, and wnt signaling as significantly altered pathways, supporting a role for Sn as intracellular signaling molecule. Real-time PCR of a subset of modulated genes confirmed these results and highlighted the reliability of a short-term cross-linking treatment for transcriptomic analysis of receptor functions.
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