Title
Follicles of various maturation stages react differently to enzymatic isolation : a comparison of different isolation protocols Follicles of various maturation stages react differently to enzymatic isolation : a comparison of different isolation protocols
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Cambridge ,
Subject
Biology
Veterinary medicine
Human medicine
Source (journal)
Reproductive biomedicine online. - Cambridge
Volume/pages
30(2015) :2 , p. 181-190
ISSN
1472-6483
ISI
000349087000012
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Isolation of human follicles is based on digestion of the tissue by combinations of enzymes. Follicle vitality and morphology are often based on the analysis of pooled follicles of different maturation stages. Information is therefore lacking on the effect of the isolation protocol to individual follicles of different maturation stages. A study was conducted using five protocols combining different enzymes and varying concentrations. Isolated follicles were classified according to their maturation stages, counted and characterized for vitality, morphology, early apoptosis and organization of transzonal projections. No statistical differences were found between the protocols when outcome parameters were analysed on a pool of follicles regardless of their maturation status. Differences were observed in quality when the follicles were analysed separately according to their maturation status. Combining morphologic characteristics and vitality, both Liberase DH and Liberase TM combined with collagenase IV were better at isolating high-quality primordial follicles, compared with collagenase IV. No statistical difference between the isolation protocols was found for primary follicles. If only high-quality isolated secondary follicles are needed, collagenase IV is found to be most advantageous. Follicles of different maturation stages react differently when enzymatic isolation protocols are compared.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/7a19cb/9445.pdf
E-info
https://repository.uantwerpen.be/docman/iruaauth/39f1a9/1999473.pdf
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