Publication
Title
Tau monoclonal antibody generation based on humanized yeast models : impact on tau oligomerization and diagnostics
Author
Abstract
A link between Tau phosphorylation and aggregation has been shown in different models for Alzheimer disease, including yeast. We used human Tau purified from yeast models to generate new monoclonal antibodies, of which three were further characterized. The first antibody, ADx201, binds the Tau proline-rich region independently of the phosphorylation status, while the second, ADx215, detects an epitope formed by the Tau N-terminus when Tau is not phosphorylated at Tyr18. For the third antibody, ADx210, the binding site could not be determined since its epitope is probably conformational. All three antibodies stained tangle-like structures in different brain sections of THY-Tau22 transgenic mice and Alzheimer patients and ADx201 and ADx210 also detected neuritic plaques in the cortex of the patient brains. In hippocampal homogenates from THY-Tau22 mice and cortex homogenates obtained from Alzheimer patients, ADx215 consistently stained specific low-order Tau oligomers in diseased brain, which in size correspond to Tau dimers. ADx201 and ADx210 additionally reacted to higher-order Tau oligomers and presumed prefibrillar structures in the patient samples. Our data further suggest that formation of low-order Tau oligomers marks an early disease stage that is initiated by Tau phosphorylation at N-terminal sites. Formation of higher-order oligomers appears to require additional phosphorylation in the C-terminus of Tau. When used to assess Tau levels in human cerebrospinal fluid, the antibodies permitted to discriminate patients with Alzheimer disease or other dementia like vascular dementia, indicative that these antibodies hold promising diagnostic potential.
Language
English
Source (journal)
Journal of biological chemistry. - Baltimore, Md
Publication
Baltimore, Md : 2015
ISSN
0021-9258
Volume/pages
290:7(2015), p. 4059-4074
ISI
000349458400018
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 30.01.2015
Last edited 05.09.2017
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