Title
Treatment with egg antigens of Schistosoma mansoni ameliorates experimental colitis in mice through a colonic T-celldependent mechanism Treatment with egg antigens of Schistosoma mansoni ameliorates experimental colitis in mice through a colonic T-celldependent mechanism
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
New York, N.Y. ,
Subject
Human medicine
Source (journal)
Inflammatory bowel diseases. - New York, N.Y.
Volume/pages
21(2015) :1 , p. 48-59
ISSN
1078-0998
ISI
000348906500011
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Background: Helminth-derived molecules are being identified as a new therapeutic approach for immune-mediated diseases. We investigated the anti-inflammatory effect and the immunological mechanisms of Schistosoma mansoni soluble egg antigens (SmSEA) in a mouse model of chronic colitis. Methods: Colitis was induced in immunocompromised severe combined immunodeficiency mice by the adoptive transfer of CD4+CD25−CD62L+ T cells. Two weeks post-transfer, SmSEA treatments were started (study 1: 1 × 20 μg SmSEA per week 5 times; study 2: 2 × 20 μg SmSEA per week 3 times). From the start of the treatment (week 2), the clinical outcome and colonic inflammation were assessed at different time points by a clinical disease score and colonoscopy, respectively. At the end of the studies, the colons were harvested for macroscopic examination, and colonic lamina propria mononuclear cells were isolated for flow cytometric T-cell characterization. Results: In both studies, administration of SmSEA in colitis mice improved all the inflammatory parameters studied. However in study 1, this beneficial effect on inflammation diminished with time, and the T-cell characterization of the lamina propria mononuclear cells, performed at week 6, revealed no immunological effects of the SmSEA treatment. In study 2, mice were killed earlier (week 4) and at that time point, we found a significant downregulation of the number of interleukin-17Aproducing T cells and a significant upregulation of the number of interleukin-4producing T cells in the colon of the SmSEA-treated colitis mice. Conclusions: Our results demonstrated that the administration of SmSEA reduces the severity of colitis in the adoptive transfer mouse model characterized by an increased Th2 response and a suppressed Th17 response in the colon.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/03c7d3/9475.pdf
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