Title Rat brain normalization templates for robust regional analysis of [$^{11}C$]ABP688 positron emission tomography/computed tomography Author Verhaeghe, Jeroen Wyffels, Leonie Wyckhuys, Tine Stroobants, Sigrid Staelens, Steven Faculty/Department Faculty of Medicine and Health Sciences Publication type article Publication 2015 2015 Subject Chemistry Biology Human medicine Computer. Automation Source (journal) Molecular imaging Volume/pages 14(2015) , 14 p. ISSN 1535-3508 ISI 000351643500009 Carrier E Target language English (eng) Full text (Publishers DOI) Affiliation University of Antwerp Abstract A methodology to generate rat brain templates for spatial normalization of positron emission tomographic (PET)/computed tomographic (CT) images is described and applied to generate three different templates for imaging of [11C]ABP688, a PET ligand binding to the metabotropic glutamate 5 receptor. The templates are based on functional (PET), structural (CT), and combined PET and CT information, respectively. The templates are created from a testretest study under normal conditions and are used to assess the different templates by using them in the analysis pipeline of a testretest and a blocking experiment. The resulting average nondisplaceable binding potentials (BPND) show significant (analysis of variance, p , .05) and substantial (up to 23%) differences between the different approaches in several brain regions. The highest BPND values in receptor-rich regions are obtained using the PET-based approach. This approach also had the smallest variability in all tested regions (standard error of measurement of 9% versus 14% [PET/CT] and 20% [CT]). All approaches showed similar relative changes in BPND values with increased blocking. Taken together, these results suggest that the use of the tracer-specific PET-based template outperforms the other approaches with the performance of the combined PET/CT template between those of the PET and the tracer-independent CT template. Full text (open access) https://repository.uantwerpen.be/docman/irua/9f35bc/1d28f52d.pdf E-info http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000351643500009&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000351643500009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848 Handle