Title
Functional characterization of the putative orphan neuropeptide G-protein coupled receptor C26F1.6 in **Caenorhabditis elegans** Functional characterization of the putative orphan neuropeptide G-protein coupled receptor C26F1.6 in **Caenorhabditis elegans**
Author
Faculty/Department
Faculty of Sciences. Chemistry
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Amsterdam ,
Subject
Biology
Source (journal)
FEBS letters: for the rapid publication of short reports in biochemistry, biophysics and molecular biology / Federation of European Biochemical Societies. - Amsterdam
Volume/pages
573(2004) , p. 55-60
ISSN
0014-5793
ISI
000223697200011
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
In this study, we describe the cloning and the characterization of the third FMRFamide-related peptide (FaRP) receptor in Caenorhabditis elegans, the VRFa receptor 1. Numerous structurally different FaRPs were synthesized and used to screen the orphan C26F1.6 receptor for activation. Two peptides ending in M(orL)VRFamide elicited a calcium response in receptor expressing mammalian cells. The response is dose-dependent and appeared to be very specific, since very closely related FaRPs were less active, even the other peptides ending in M(orL)VRFamide. Pharmacological profiling of the most active peptide suggests that SMVRFa is the most active binding core. N-terminal extension decreases peptide activity.
E-info
https://repository.uantwerpen.be/docman/iruaauth/21383d/5a69d446be1.pdf
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