Publication
Title
Characterization of an RFamide-related peptide orphan GPCR in **C. elegans**
Author
Abstract
We cloned and characterized an orphan FMRFamide-related peptide (FaRP) GPCR in Caenorhabditis elegans. We synthesized numerous structurally different FaRPs that were found in the C. elegans genome by bioinformatic analysis and used them to screen cells expressing the C26F1.6 receptor. Two peptides ending in M(orL)VRFamide elicited a calcium response in receptor-expressing mammalian Chinese hamster ovary cells. The response was dose-dependent and appeared to be very specific; that is, none of the other FaRPs were active, not even closely related peptides also ending in M(orL)VRFamide, which are encoded by the same peptide precursor. Pharmacological profiling with a truncated series of the most active peptide revealed that the full peptide sequence is necessary for receptor activation.
Language
English
Source (journal)
Annals of the New York Academy of Sciences / New York Academy of Sciences. - New York, N.Y.
Publication
New York, N.Y. : 2005
ISSN
0077-8923
Volume/pages
1040(2005), p. 410-412
ISI
000230936100075
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
[E?say:metaLocaldata.cgzprojectinf]
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 05.02.2015
Last edited 17.08.2017