Title
Characterization of an RFamide-related peptide orphan GPCR in **C. elegans**
Author
Faculty/Department
Faculty of Sciences. Chemistry
Faculty of Medicine and Health Sciences
Publication type
article
Publication
New York, N.Y. ,
Subject
Biology
Source (journal)
Annals of the New York Academy of Sciences / New York Academy of Sciences. - New York, N.Y.
Volume/pages
1040(2005) , p. 410-412
ISSN
0077-8923
ISI
000230936100075
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
We cloned and characterized an orphan FMRFamide-related peptide (FaRP) GPCR in Caenorhabditis elegans. We synthesized numerous structurally different FaRPs that were found in the C. elegans genome by bioinformatic analysis and used them to screen cells expressing the C26F1.6 receptor. Two peptides ending in M(orL)VRFamide elicited a calcium response in receptor-expressing mammalian Chinese hamster ovary cells. The response was dose-dependent and appeared to be very specific; that is, none of the other FaRPs were active, not even closely related peptides also ending in M(orL)VRFamide, which are encoded by the same peptide precursor. Pharmacological profiling with a truncated series of the most active peptide revealed that the full peptide sequence is necessary for receptor activation.
E-info
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