Accelerating effect of hydroxylamine and hydrazine on nitrogen removal rate in moving bed biofilm reactor
Faculty of Sciences. Bioscience Engineering
Engineering sciences. Technology
Biodegradation. - Dordrecht
, p. 739-749
In biological nitrogen removal, application of the autotrophic anammox process is gaining ground worldwide. Although this field has been widely researched in last years, some aspects as the accelerating effect of putative intermediates (mainly N2H4 and NH2OH) need more specific investigation. In the current study, experiments in a moving bed biofilm reactor (MBBR) and batch tests were performed to evaluate the optimum concentrations of anammox process intermediates that accelerate the autotrophic nitrogen removal and mitigate a decrease in the anammox bacteria activity using anammox (anaerobic ammonium oxidation) biomass enriched on ring-shaped biofilm carriers. Anammox biomass was previously grown on blank biofilm carriers for 450 days at moderate temperature 26.0 (+/- 0.5) A degrees C by using sludge reject water as seeding material. FISH analysis revealed that anammox microorganisms were located in clusters in the biofilm. With addition of 1.27 and 1.31 mg N L-1 of each NH2OH and N2H4, respectively, into the MBBR total nitrogen (TN) removal efficiency was rapidly restored after inhibitions by NO2 (-). Various combinations of N2H4, NH2OH, NH4 (+), and NO2 (-) were used as batch substrates. The highest total nitrogen (TN) removal rate with the optimum N2H4 concentration (4.38 mg N L-1) present in these batches was 5.43 mg N g(-1) TSS h(-1), whereas equimolar concentrations of N2H4 and NH2OH added together showed lower TN removal rates. Intermediates could be applied in practice to contribute to the recovery of inhibition-damaged wastewater treatment facilities using anammox technology.