Title
Reactivation of aerobic and anaerobic ammonium oxidizers in OLAND biomass after long-term storage Reactivation of aerobic and anaerobic ammonium oxidizers in OLAND biomass after long-term storage
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Berlin ,
Subject
Biology
Engineering sciences. Technology
Source (journal)
Applied microbiology and biotechnology. - Berlin, 1984, currens
Volume/pages
74(2007) :6 , p. 1376-1384
ISSN
0175-7598
ISI
000245896800024
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The biomass of an oxygen-limited autotrophic nitrification/denitrification (OLAND) biofilm reactor was preserved in various ways to find a storage method for both aerobic and anaerobic ammonium-oxidizing bacteria (AerAOB and AnAOB). Storage occurred at -20 degrees C with and without glycerol as cryoprotectant and at 4 and -20 degrees C with and without nitrate as redox buffer. After 2 and 5 months, reactivation of AerAOB and AnAOB was achieved with the biomass stored at 4 degrees C with and without nitrate and at 20 degrees C with nitrate. Moreover, the presence of the AerAOB and AnAOB was confirmed with fluorescent in situ hybridization (FISH). Preservation in a nitrate environment resulted in a lag phase for the AnAOB reactivation. The supplied nitrate was denitrified during storage, and a real-time polymerase chain reaction with nitrifying and denitrifying genes allowed to estimate that at least 1.0 to 6.0% of the OLAND biofilm consisted of denitrifiers. It was concluded that reactivation after longterm storage is possible and that preservation at 4 degrees C without nitrate addition is the recommended storage technique. The possibility to store OLAND biomass will facilitate research on AnAOB and can overcome largerscale start-up and inhibition problems of novel nitrogen processes involving AnAOB.
E-info
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