Title
Physico-chemical characterization of exopolysaccharides of potential probiotic enterococcus faecium isolates from infants'gut Physico-chemical characterization of exopolysaccharides of potential probiotic enterococcus faecium isolates from infants'gut
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Subject
Biology
Engineering sciences. Technology
Source (journal)
Current biochemical engineering
Volume/pages
(2015)
ISSN
2212-7119
Carrier
E
Target language
Dutch (dut)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Microbial exopolysaccharides (EPS) may have technological applications in food and pharmaceutical industries, and in environmental health. EPS may be used in foods as natural thickening, stabilizing, bodying or gelling agent, and/or as emulsifiers or as fat replacer. In addition, EPS has been reported to have health augmenting features such as prebiotic, immunostimulatory, anti-tumor and hypocholesterolemic properties. EPS producing probiotics bacteria may help enhancing organoleptic properties of fermented foods. In present study, Enterococcus faecium strains viz. Enterococcus faecium 1.1, E. faecium 2.0 and E. faecium 4.0, which had several probiotically important attributes, were investigated for their EPS producing potential. E. faecium 4.0 produced highest yield of EPS (470 mg/L) and was followed by E. faecium 1.1 (220 mg/L) and E. faecium 2.0 (180 mg/L). FTIR analysis showed that EPS from all the three E. faecium strains was homopolymer of glucose. Degradation temperatures of EPS from three E. faecium strains varied in the range of 270.4°C-279.3°C as studied by thermal gravimetric analysis. Differential scanning calorimetry of EPS from E. faecium 1.1, E. faecium 2.0 and E. faecium 4.0 showed the melting point of 55.9°C, 88.6°C and 83.5°C; and enthalpy of 102.5, 56.03, and 213.9 J/g, respectively. EPS from all the three E. faecium strains expressed growth inhibition of E. coli. Purified EPS from all three E. faecium strains exhibited excellent emulsification properties i.e. 96.70 % (E. faecium 1.1), 96.78 % (E. faecium 2.0) and 97.77 % (E. faecium 4.0). - See more at: http://www.eurekaselect.com/123943/article#sthash.wbtJ9ZLb.dpuf
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