In vivo visualization and quantification of mitochondrial morphology in **C. elegans**In vivo visualization and quantification of mitochondrial morphology in **C. elegans**
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Laboratory of cell biology and histology
Mitochondrial Medicine, Volume II Manipulating Mitochondrial Function/ Weissig, Volkmar (Edit.), e.a.
Methods in molecular biology. - Totowa, N.J., 1984, currens; -
University of Antwerp
Caenorhabditis elegans is a highly malleable model system, intensively used for functional, genetic, cytometric and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology.