Publication
Title
Fluorescent activated cell sorting : an effective approach to study dendritic cell subsets in human atherosclerotic plaques
Author
Abstract
Different immune cell types are present within atherosclerotic plaques. Dendritic cells (DC) are of special interest, since they are considered as the center of the immuniverse. Identifying inflammatory DC subtypes within plaques is important for a better understanding of the lesion pathogenesis and pinpoints their contribution to the atherosclerotic process. We have developed a flow cytometry-based method to characterize and isolate different DC subsets (i.e. CD11b+, Clec9A+ and CD16+ conventional (c)DC and CD123+ plasmacytoid (p)DC) in human atherosclerotic plaques. We revealed a predominance of pro-inflammatory CD11b+ DC in advanced human lesions, whereas atheroprotective Clec9A+ DC were almost absent. CD123+ pDC and CD16+ DC were also detectable in plaques. Remarkably, plaques from distinct anatomical locations exhibited different cellular compositions: femoral plaques contained less CD11b+ and Clec9A+ DC than carotid plaques. Twice as many monocytes/macrophages were observed compared to DC. Moreover, relative amounts of T cells/B cells/NK cells were 6 times as high as DC numbers. For the first time, fluorescent activated cell sorting analysis of DC subsets in human plaques indicated a predominance of CD11b+ cDC, in comparison with other DC subsets. Isolation of the different subsets will facilitate detailed functional analysis and may have significant implications for tailoring appropriate therapy.
Language
English
Source (journal)
Journal of immunological methods. - Amsterdam
Publication
Amsterdam : 2015
ISSN
0022-1759
Volume/pages
417(2015), p. 76-85
ISI
000352046500009
Full text (Publishers DOI)
Full text (open access)
Full text (publishers version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 09.03.2015
Last edited 21.04.2017
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