Publication
Title
Characterization of the peripheral blood transcriptome in a repeated measures design using a panel of healthy individuals
Author
Abstract
A repeated measures microarray design with 22 healthy, non-smoking volunteers (aging 32 ± 5 years) was set up to study transcriptome profiles in whole blood samples. The results indicate that repeatable data can be obtained with high within-subject correlation. Probes that could discriminate between individuals are associated with immune and inflammatory functions. When investigating possible time trends in the microarray data, we have found no differential expression within a sampling period (within-season effect). Differential expression was observed between sampling seasons and the data suggest a weak response of genes related to immune system functioning. Finally, a high number of probes showed significant season-specific expression variability within subjects. Expression variability increased in springtime and there was an association of the probe list with immune system functioning. Our study suggests that the blood transcriptome of healthy individuals is reproducible over a time period of several months.
Language
English
Source (journal)
Genomics: international journal of gene mapping and nucleotide sequencing. - San Diego, Calif.
Publication
San Diego, Calif. : 2014
ISSN
0888-7543
Volume/pages
103:1(2014), p. 31-39
ISI
000333512800004
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 21.03.2015
Last edited 19.10.2017
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