Publication
Title
A hybrid, de novo based, genome-wide database search approach applied to the sea urchin neuropeptidome
Author
Abstract
Peptidomics is the identification and study of the in vivo biologically active peptide profile. A combination of high performance liquid chromatography, mass spectrometry, and bioinformatics tools such as database search engines are commonly used to perform the analysis. We report a methodology based on a database system holding the completed translated genome, whereby de novo sequencing and genome-wide database searching are combined. The methodology was applied to the sea urchin neuropeptidome resulting in a 30% increase in identification rate.
Language
English
Source (journal)
Journal of proteome research
Publication
2010
ISSN
1535-3893
Volume/pages
9:2(2010), p. 990-996
ISI
000274269400035
Full text (Publishers DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 25.03.2015
Last edited 23.05.2017