Title
A hybrid, de novo based, genome-wide database search approach applied to the sea urchin neuropeptidome A hybrid, de novo based, genome-wide database search approach applied to the sea urchin neuropeptidome
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Subject
Chemistry
Biology
Source (journal)
Journal of proteome research
Volume/pages
9(2010) :2 , p. 990-996
ISSN
1535-3893
ISI
000274269400035
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Peptidomics is the identification and study of the in vivo biologically active peptide profile. A combination of high performance liquid chromatography, mass spectrometry, and bioinformatics tools such as database search engines are commonly used to perform the analysis. We report a methodology based on a database system holding the completed translated genome, whereby de novo sequencing and genome-wide database searching are combined. The methodology was applied to the sea urchin neuropeptidome resulting in a 30% increase in identification rate.
E-info
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