Title
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Degradation profile of []-corazonin in the hemolymph of the desert locust **Schistocerca gregaria**
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Author
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Abstract
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Degradation of the neuropeptide [His(7)]-corazonin, a key hormone in phase transition in locusts was studied using [H-3] [His(7)] -corazonin, RP-HPLC and mass spectrometry. After 4 h incubation, 50 and 75% of [His(7)]-corazonin could still be found in hemolymph of gregarious and solitarious Schistocerca gregaria, respectively. Under in vivo conditions the half-life was 30 min. These results are in contrast to many other neuropeptides that usually have half lives of a few minutes. The peptide is cleaved first by an endopeptidase, either just before or after the Tyr residue at position 5. Next, the C-terminal degradation fragments are further degraded by a dipeptidyl-peptidase, whereas the N-terminal fragments are further broken down one amino acid at a time. In addition, [Dopa(5)][His(7)]-corazonin was detected. Upon synthesis, this unexpected molecular modification turned out to be biologically active in bringing about cuticular melanization. (c) 2005 Elsevier Inc. All rights reserved. |
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Language
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English
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Source (journal)
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Peptides. - Fayetteville, N.Y.
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Publication
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Fayetteville, N.Y.
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2006
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ISSN
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0196-9781
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DOI
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10.1016/J.PEPTIDES.2005.08.021
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Volume/pages
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27
:3
(2006)
, p. 539-548
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ISI
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000236230200009
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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