Title
Degradation profile of [<tex>$His^{7}$</tex>]-corazonin in the hemolymph of the desert locust **Schistocerca gregaria** Degradation profile of [<tex>$His^{7}$</tex>]-corazonin in the hemolymph of the desert locust **Schistocerca gregaria**
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Fayetteville, N.Y. ,
Subject
Chemistry
Biology
Pharmacology. Therapy
Source (journal)
Peptides. - Fayetteville, N.Y.
Volume/pages
27(2006) :3 , p. 539-548
ISSN
0196-9781
ISI
000236230200009
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Degradation of the neuropeptide [His(7)]-corazonin, a key hormone in phase transition in locusts was studied using [H-3] [His(7)] -corazonin, RP-HPLC and mass spectrometry. After 4 h incubation, 50 and 75% of [His(7)]-corazonin could still be found in hemolymph of gregarious and solitarious Schistocerca gregaria, respectively. Under in vivo conditions the half-life was 30 min. These results are in contrast to many other neuropeptides that usually have half lives of a few minutes. The peptide is cleaved first by an endopeptidase, either just before or after the Tyr residue at position 5. Next, the C-terminal degradation fragments are further degraded by a dipeptidyl-peptidase, whereas the N-terminal fragments are further broken down one amino acid at a time. In addition, [Dopa(5)][His(7)]-corazonin was detected. Upon synthesis, this unexpected molecular modification turned out to be biologically active in bringing about cuticular melanization. (c) 2005 Elsevier Inc. All rights reserved.
E-info
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