Publication
Title
Degradation profile of []-corazonin in the hemolymph of the desert locust **Schistocerca gregaria**
Author
Abstract
Degradation of the neuropeptide [His(7)]-corazonin, a key hormone in phase transition in locusts was studied using [H-3] [His(7)] -corazonin, RP-HPLC and mass spectrometry. After 4 h incubation, 50 and 75% of [His(7)]-corazonin could still be found in hemolymph of gregarious and solitarious Schistocerca gregaria, respectively. Under in vivo conditions the half-life was 30 min. These results are in contrast to many other neuropeptides that usually have half lives of a few minutes. The peptide is cleaved first by an endopeptidase, either just before or after the Tyr residue at position 5. Next, the C-terminal degradation fragments are further degraded by a dipeptidyl-peptidase, whereas the N-terminal fragments are further broken down one amino acid at a time. In addition, [Dopa(5)][His(7)]-corazonin was detected. Upon synthesis, this unexpected molecular modification turned out to be biologically active in bringing about cuticular melanization. (c) 2005 Elsevier Inc. All rights reserved.
Language
English
Source (journal)
Peptides. - Fayetteville, N.Y.
Publication
Fayetteville, N.Y. : 2006
ISSN
0196-9781
Volume/pages
27:3(2006), p. 539-548
ISI
000236230200009
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 25.03.2015
Last edited 07.10.2017