Title
SIFamide is a highly conserved neuropeptide : a comparative study in different insect species SIFamide is a highly conserved neuropeptide : a comparative study in different insect species
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York, N.Y. ,
Subject
Physics
Chemistry
Biology
Source (journal)
Biochemical and biophysical research communications. - New York, N.Y.
Volume/pages
320(2004) :2 , p. 334-341
ISSN
0006-291X
ISI
000222567700008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Neb-LFamide or AYRKPPFNGSLFamide was originally purified from the grey flesh fly Neobellieria bullata as a myotropic neuropeptide. We studied the occurrence of this peptide and its isoforms in the central nervous system of different insect species by means of whole mount fluorescence immunohistochemistry, mass spectrometry, and data mining. We found that both sequence and immunoreactive distribution pattern are very conserved in the studied insects. In all species and stages we counted two pairs of immunoreactive cells in the pars intercerebralis. These cells projected axons throughout the ventral nerve cord. In the adult CNSs they formed a large number of immunoreactive varicosities as well. Mass spectrometry and data mining revealed that SIFamide exists in two isoforms: [G(1)]-SIFamide and [A(1)]-SIFamide. In addition, the SIFamide joining peptide is relatively well conserved throughout arthropod species. The conserved presence of two cysteine residues, separated by six amino acid residues, allows the formation of disulphide bridges. (C) 2004 Elsevier Inc. All rights reserved.
E-info
https://repository.uantwerpen.be/docman/iruaauth/b81b7d/c8b9655.pdf
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