Neuropeptidomics of the grey flesh fly, **Neobellieria bullata**

Verleyen, P.; Huybrechts, J.; Sas, F.; Clynen, E.; Baggerman, G.; De Loof, A.; Schoofs, L.

doi:10.1016/J.BBRC.2004.02.115

Title

Neuropeptidomics of the grey flesh fly, **Neobellieria bullata**

Author

Verleyen, P.

Huybrechts, J.

Sas, F.

Clynen, E.

Baggerman, G.

De Loof, A.

Schoofs, L.

Abstract

A peptidomics approach was applied to determine the peptides in the larval central nervous system of the grey flesh fly, Neo-bellieria bullata. Fractions obtained by high performance liquid chromatography were analysed by MALDI-TOF and ESI-Q-TOF mass spectrometry. This provided biochemical evidence for the presence of 18 neuropeptides, 11 of which were novel Neobellieria peptides. Most prominently present were the FMRFamide-related peptides: 7 FNIRFamides, 1 FIRFamide, and Neb-myosuppressin. The three putative capa-gene products Neb-pyrokinin and the periviscerokinins Neb-PVK-1 and -2 were detected, as well as another pyrokinin. This Neb-PK-2 was also present in the ring gland along with corazonin, Neb-myosuppressin, and Neb-AKH-GK, an intermediate processing product of the adipokinetic hormone. Furthermore, the central nervous system contained Neb-LFamide, proctolin, and FDFHTVamide, designated as Neb-TVamide. With this study, we considerably increased our knowledge of the neuropeptidome of the pest fly N. bullata, which is an important insect model for physiological research. (C) 2004 Elsevier Inc. All rights reserved.

Language

English

Source (journal)

Biochemical and biophysical research communications. - New York, N.Y.

Publication

New York, N.Y. : 2004

ISSN

0006-291X

DOI

10.1016/J.BBRC.2004.02.115

Volume/pages

316 :3 (2004) , p. 763-770