Title
Proteomics in **Drosophila melanogaster** : first 2D database of larval hemolymph proteins Proteomics in **Drosophila melanogaster** : first 2D database of larval hemolymph proteins
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York, N.Y. ,
Subject
Physics
Chemistry
Biology
Source (journal)
Biochemical and biophysical research communications. - New York, N.Y.
Volume/pages
304(2003) :4 , p. 831-838
ISSN
0006-291X
ISI
000182912000040
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
A proteomic approach was used for the identification of larval hemolymph proteins of Drosophila melanogaster. We report the initial establishment of a two-dimensional gel electrophoresis reference map for hemolymph proteins of third instar larvae of D. melanogaster. We used immobilized pH gradients of pH 4-7 (linear) and a 12-14% linear gradient polyacrylamide gel. The protein spots were silver-stained and analyzed by nanoLC-Q-Tof MS/MS (on-line nanoscale liquid chromatography quadrupole time of flight tandem mass spectrometry) or by Matrix assisted laser desorption time of flight MS (MALDI-TOF MS). Querying the SWISSPROT database with the mass spectrometric data yielded the identity of the proteins in the spots. The presented proteome map lists those protein spots identified to date. This map will be updated continuously and will serve as a reference database for investigators, studying changes at the protein level in different physiological conditions. (C) 2003 Elsevier Science (USA). All rights reserved.
E-info
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