Proteomics in **Drosophila melanogaster** : first 2D database of larval hemolymph proteins

Vierstraete, E.; Cerstiaens, A.; Baggerman, G.; Van den Bergh, G.; De Loof, A.; Schoofs, L.

doi:10.1016/S0006-291X(03)00683-1

Title

Proteomics in **Drosophila melanogaster** : first 2D database of larval hemolymph proteins

Author

Vierstraete, E.

Cerstiaens, A.

Baggerman, G.

Van den Bergh, G.

De Loof, A.

Schoofs, L.

Abstract

A proteomic approach was used for the identification of larval hemolymph proteins of Drosophila melanogaster. We report the initial establishment of a two-dimensional gel electrophoresis reference map for hemolymph proteins of third instar larvae of D. melanogaster. We used immobilized pH gradients of pH 4-7 (linear) and a 12-14% linear gradient polyacrylamide gel. The protein spots were silver-stained and analyzed by nanoLC-Q-Tof MS/MS (on-line nanoscale liquid chromatography quadrupole time of flight tandem mass spectrometry) or by Matrix assisted laser desorption time of flight MS (MALDI-TOF MS). Querying the SWISSPROT database with the mass spectrometric data yielded the identity of the proteins in the spots. The presented proteome map lists those protein spots identified to date. This map will be updated continuously and will serve as a reference database for investigators, studying changes at the protein level in different physiological conditions. (C) 2003 Elsevier Science (USA). All rights reserved.

Language

English

Source (journal)

Biochemical and biophysical research communications. - New York, N.Y.

Publication

New York, N.Y. : 2003

ISSN

0006-291X

DOI

10.1016/S0006-291X(03)00683-1

Volume/pages

304 :4 (2003) , p. 831-838