Title
Isolation of angiotensin converting enzyme from testes of **Locusta migratoria** (Orthoptera) Isolation of angiotensin converting enzyme from testes of **Locusta migratoria** (Orthoptera)
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Subject
Biology
Source (journal)
European journal of entomology / Euromonitor
Volume/pages
100(2003) :4 , p. 467-474
ISSN
1210-5759
ISI
000187974700002
Carrier
E
Target language
English (eng)
Abstract
By means of a tracer assay using a labeled synthetic angiotensin converting enzyme (ACE) substrate hippurylglycylglycine, we have detected high ACE activity in the testes of the African migratory locust, Locusta migratoria. Lower, but significant, ACE activity was observed in midgut and hemolymph. In a two-step purification procedure involving anion exchange and gel permeation chromatography, we have purified LomACE from the locust testes. The enzyme of approximately 80 kDa shows substantial amino-acid sequence homology with ACE from both vertebrate and invertebrate origin. The ACE identity of the purified enzyme was further confirmed by cDNA cloning of the Locusta ACE fragment, which, after in silico translation, revealed a mature protein of 623 amino acids with a large structural similarity to other known ACE proteins.
E-info
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