Title
Isolation, identification, and synthesis of a disulfated sulfakinin from the central nervous system of an arthropod, the white shrimp **Litopenaeus vannamei** Isolation, identification, and synthesis of a disulfated sulfakinin from the central nervous system of an arthropod, the white shrimp **Litopenaeus vannamei**
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York, N.Y. ,
Subject
Physics
Chemistry
Biology
Source (journal)
Biochemical and biophysical research communications. - New York, N.Y.
Volume/pages
299(2002) :2 , p. 312-320
ISSN
0006-291X
ISI
000179515900025
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Two myotropic peptides displaying tyrosyl sulfation have been isolated from an extract of central nervous systems (brain, suboesophageal ganglion, thoracic ganglia, and ventral nerve cord) of the white shrimp Litopenaeus cannamei, Both peptides were identified by mass spectrometry and belong to the sulfakinin family of neuropeptides, which are characterized by the C-terminal hexapeptide Y(SO3H)GHMRF-NH2 preceded by two acidic amino acid residues, Pev-SK1 (AGGSGGVGGEY(SO3H)DDY (SO3H)GH(L/I) RF-NH2) has two sulfated tyrosyl residues and a unique (L/I) for M substitution in the C-terminal sequence. Pev-SK 2 (pQFDEY(SO3H)GHMRF-NH2) fully complies with the typical sulfakinin Core sequence and is blocked by a pyroglutamyl residue. Synthetic analogs (sulfated and unsulfated) were synthesized and the tyrosyl sulfations were confirmed by myotropic activity studies and co-elution with the native fractions. Pev-SK I is the first disulfated neuropeptide elucidated in the phylum of the arthropoda, with the only other reported disulfated neuropeptide. called cionin. found in a protochordate. The similarities in amino acid sequence and posttranslational modifications of the crustacean sulfakinins and protochordate cionin provide further evidence for the hypothesis stating that gastrin/CCK. cionin, and sulfakinins originate from a common ancestral gastrin/CCK-like peptide. (C) 2002 Elsevier Science (USA). All rights reserved.
E-info
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