Title
Cold stress alters **Mytilus edulis** pedal ganglia expression of <tex>$\mu$</tex> opiate receptor transcripts determined by real-time RT-PCR and morphine levels Cold stress alters **Mytilus edulis** pedal ganglia expression of <tex>$\mu$</tex> opiate receptor transcripts determined by real-time RT-PCR and morphine levels
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Amsterdam ,
Subject
Human medicine
Source (journal)
Molecular brain research. - Amsterdam
Volume/pages
99(2002) :1 , p. 26-33
ISSN
0169-328X
ISI
000174472500004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Previous pharmacological, biochemical and molecular evidence prove that mu-subtype opiate receptors and opiate alkaloids, i.e. morphine, are present in the ganglionic nervous system of the mollusk Mytilus edulis (bivalve). We now present molecular evidence on the effect of rapid temperature changes on mu opiate receptor expression and morphine levels. Using primers, a labeled Taq-Man probe derived from the human neuronal mu1 opiate receptor, and real-time RT-PCR to measure the expression of mu transcripts from Mytilus pedal ganglia, we observe, in animals placed in cold water from room temperature. an enhanced morphine and morphine 6 glucuronide level in addition to a decrease in mu opiate receptor gene expression. This study provides further evidence that mu-type opiate receptors and morphine are expressed in mollusk ganglia and appear to be involved in physiological processes responding to thermal stress. (C) 2002 Elsevier Science BV All rights reserved.
E-info
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