Publication
Title
Cold stress alters **Mytilus edulis** pedal ganglia expression of opiate receptor transcripts determined by real-time RT-PCR and morphine levels
Author
Abstract
Previous pharmacological, biochemical and molecular evidence prove that mu-subtype opiate receptors and opiate alkaloids, i.e. morphine, are present in the ganglionic nervous system of the mollusk Mytilus edulis (bivalve). We now present molecular evidence on the effect of rapid temperature changes on mu opiate receptor expression and morphine levels. Using primers, a labeled Taq-Man probe derived from the human neuronal mu1 opiate receptor, and real-time RT-PCR to measure the expression of mu transcripts from Mytilus pedal ganglia, we observe, in animals placed in cold water from room temperature. an enhanced morphine and morphine 6 glucuronide level in addition to a decrease in mu opiate receptor gene expression. This study provides further evidence that mu-type opiate receptors and morphine are expressed in mollusk ganglia and appear to be involved in physiological processes responding to thermal stress. (C) 2002 Elsevier Science BV All rights reserved.
Language
English
Source (journal)
Molecular brain research. - Amsterdam
Publication
Amsterdam : 2002
ISSN
0169-328X
Volume/pages
99:1(2002), p. 26-33
ISI
000174472500004
Full text (Publishers DOI)
Full text (publishers version - intranet only)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 25.03.2015
Last edited 22.05.2017