Title
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Crustacean hyperglycaemic hormone (CHH)-like peptides and CHH-precursor-related peptides from pericardial organ neurosecretory cells in the shore crab, **Carcinus maenas**, are putatively spliced and modified products of multiple genes
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Author
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Abstract
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About 24 intrinsic neurosecretory neurons within the pericardial organs (POs) of the crab Carcinus maenas produce a novel crustacean hyperglycaemic hormone (CHH)-like peptide (PO-CHH) and two CHH-precursor-related peptides (PO-CPRP I and II) as identified immunochemically and by peptide chemistry. Edman sequencing and MS revealed PO-CHH as a 73 amino acid peptide (8630 Da) with a free C-terminus, PO-CHH and sinus gland CHH (SG-CHH) share an identical N-terminal sequence, positions 1-40, but the remaining sequence, positions 41-73 or 41-72, differs considerably. PO-CHH may have different precursors, as cDNA cloning of PO-derived mRNAs has revealed several similar forms, one exactly encoding the peptide. All PO-CHH cDNAs contain a nucleotide stretch coding fur the SG-CHH41-76 sequence in the 3'-untranslated region (UTR). Cloning of crab testis genomic DNA revealed at least four CHH genes, the structure of which suggest that PO-CHH and SG-CHH arise by alternative splicing of precursors acid possibly post-transcriptional modification of PO-CHH. The genes encode four exons, separated by three variable introns, encoding part of a signal peptide (exon I), the remaining signal peptide residues. a CPRP, the PO-CHH1-40/SG-CHH1-40 sequences (exon II), the remaining PO-CHH residues (exon III) and the remaining SG-CHH residues acid a 3'-UTR (exon IV), Precursor and gene structures are more closely related to those encoding related insect ion-transport peptides than to penaeid shrimp CHH genes. PO-CHH neither exhibits hyperglycaemic activity in vivo, nor does it inhibit Y-organ ecdysteroid synthesis in vitro. From the morphology of the neurons it seems likely that novel functions remain to be discovered. |
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Language
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English
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Source (journal)
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The biochemical journal. - London, 1984, currens
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Publication
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London
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2001
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ISSN
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0264-6021
[print]
1470-8728
[online]
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DOI
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10.1042/0264-6021:3560159
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Volume/pages
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356
:1
(2001)
, p. 159-170
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ISI
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000169056200019
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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