Title
Presence of morphine and morphine-6-glucuronide in the marine mollusk **Mytilus edulis** ganglia determined by GC/MS and Q-TOF-MS - Starvation increases opiate alkaloid levels Presence of morphine and morphine-6-glucuronide in the marine mollusk **Mytilus edulis** ganglia determined by GC/MS and Q-TOF-MS - Starvation increases opiate alkaloid levels
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Amsterdam ,
Subject
Human medicine
Source (journal)
Molecular brain research. - Amsterdam
Volume/pages
88(2001) :1-2 , p. 155-160
ISSN
0169-328X
ISI
000168164400015
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Morphine and morphine-6-glucuronide, a morphine metabolite, have been identified and quantified in Mytilus edulis pedal ganglia at a level of 2.67 +/-0.44 and 0.98 +/-0.14 ng/ganglia, respectively by high performance liquid chromatography coupled to electrochemical detection. These opiate alkaloids were further identified by both gas-chromatography mass spectrometry and nanoflow electrospray ionization double quadrupole orthogonal acceleration Time of Flight mass spectrometry. In animals that were starved, the morphine level rose to 6.38 +/-0.88 ng/ganglion and the morphine 6-glucoronide rose to a level of 23.0 +/-3.2 ng/ganglion after 30 days. These studies demonstrate that opiate alkaloids are present as naturally occurring signal molecules whose levels respond to stress, i.e., starvation. Opiate alkaloids were not found in the animal's incubation media or food, demonstrating their synthesis occurred in the respective tissue. These new method of opiate alkaloid detection, conclusively proves that morphine and morphine-6-glucuronide are present in animal tissues. (C) 2001 Elsevier Science B.V. All rights reserved.
E-info
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