Publication
Title
Identification of morphine and morphine-6-glucuronide in the adrenal medullary chromaffin PC-12 cell line by nano-electrospray ionization double quadrupole orthogonal-acceleration time-of-flight mass spectrometry
Author
Abstract
Using nano-electrospray ionization double quadrupole orthogonal-acceleration time-of-flight mass spectrometry, we were able to detect sub-nanogram levels of endogenous morphine and its metabolite morphine-6-glucuronide in the rat pheochromocytoma cell line. Furthermore, fragmentation of endogenous morphine-6-glucuronide (462.14 Da) by collision-induced dissociation yields morphine (286.14 Da) obtained from the cell samples and with the authentic material, further demonstrating the presence of endogenous morphine. The incubation medium was devoid of this material. This methodology represents a sensitive and accurate method to detect trace amounts of endogenous opiates at subnanogram levels in biological samples.
Language
English
Source (journal)
European journal of mass spectrometry. - Chichester :
Publication
Chichester : : IM Publications,, 2001
ISSN
1469-0667
Volume/pages
7:1(2001), p. 25-28
ISI
000169599700005
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 25.03.2015
Last edited 20.06.2017