Title
Identification of novel periviscerokinins from single neurohaemal release sites in insects : MS/MS fragmentation complemented by Edman degradation
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Berlin ,
Subject
Chemistry
Biology
Source (journal)
European journal of biochemistry. - Berlin
Volume/pages
267(2000) :12 , p. 3869-3873
ISSN
0014-2956
ISI
000087624600053
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Three novel members of the periviscerokinin family could be identified directly from extracts of single abdominal perisympathetic organs of blaberoid cockroaches by means of electrospray ionization-quadrupole time of flight (ESI-QTOF) MS. Sequences of these periviscerokinins were confirmed by Edman degradation. Their primary structures are GSSGLIPFGRT-NH2 (Lem-PVK-1), GSSGLISMPRV-NH2 (Lem-PVK-2), and GSSGMIPFPRV-NH2 (Lem-PVK-3). Hitherto only known from the American cockroach, this neuropeptide family contains a highly conserved N-terminus whereas, at the C-terminus, only the penultimate amino-acid residue (Arg) has been found in all members of this peptide family. The identified periviscerokinins are the only abundant myoactive peptides in abdominal perisympathetic organs of blaberoid cockroches and they appear to be absent in the retrocerebral complex. Screening of extracts of single abdominal perisympathetic organs (70-90 mu m in diameter), from five different species of the suborder Blaberoidea, revealed that they all contain the three neuropeptides which are described here for the first time.
E-info
https://repository.uantwerpen.be/docman/iruaauth/8f64a1/efa9689.pdf
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