Title
Identification of a new tachykinin from the midgut of the desert locust, **Schistocerca gregaria**, by ESI-Qq-oa-TOF mass spectrometry Identification of a new tachykinin from the midgut of the desert locust, **Schistocerca gregaria**, by ESI-Qq-oa-TOF mass spectrometry
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York, N.Y. ,
Subject
Physics
Chemistry
Biology
Source (journal)
Biochemical and biophysical research communications. - New York, N.Y.
Volume/pages
266(1999) :1 , p. 237-242
ISSN
0006-291X
ISI
000084140800041
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
This paper reports the purification of a tachykinin isoform from the midgut of the desert locust, Schistocerca gregaria. One hundred locust midguts were extracted in an acidified methanolic solvent, after which three HPLC column systems were used to obtain a pure peptide. A tachykinin immunoassay was used to monitor all collected fractions. After each purification step the purity of the sample was monitored by MALDI-TOF mass spectrometry. The pure peptide was sequenced by ESI-Qq-oa-TOF mass spectrometry. Ed-man degradation-based automated microsequencing and chemical synthesis confirmed the sequences. The midgut peptide, GNTKKAVPGFYGTRamide (Scg-midgut-TK), belongs to the tachykinin family with identified members in all vertebrate phyla and some invertebrate phyla: arthropods, annelids and molluscs. Scg-midgut-TK is the first tachykinin purified from midguts of the desert locust Schistocerca gregaria. In comparison to locust brain tachykinins, the midgut tachykinin is N-terminally extended. Similar to neuropeptide gamma, an N-terminally extended mammalian tachykinin, first isolated from rabbit intestine, the present identified locust intestinal tachykinin contains a putative dibasic cleavage site. (C) 1999 Academic Press.
E-info
https://repository.uantwerpen.be/docman/iruaauth/d1e228/d369691.pdf
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