Publication
Title
Markov-chain-based heteroscedastic regression model for the analysis of high-resolution enzymatically O-18-labeled mass spectra
Author
Abstract
The enzymatic O-18-labeling is a useful technique for reducing the influence of the between-spectrum variability on the results of mass-spectrometry experiments. A limitation of the technique is the possibility of an incomplete labeling, which may result in biased estimates of the relative peptide abundance. We propose a Markov-chain-based regression model with heterogeneous residual variance, which corrects for the possible bias. Our method does not require extra experimental steps, as proposed in the approaches proposed previously in the literature. On the other hand, it includes some of the alternative approaches as a special case. Moreover, our modeling approach offers additional advantages over the previously developed methods, including the possibility of the analysis of multiple technical replicates for samples from different biological conditions, with an assessment of the between-spectra and biological variability.
Language
English
Source (journal)
Journal of proteome research
Publication
2010
ISSN
1535-3893
Volume/pages
9:5(2010), p. 2669-2677
ISI
000277353200055
Full text (Publishers DOI)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 25.03.2015
Last edited 23.05.2017