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Title
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The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality
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Author
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Abstract
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| In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity (obese) and a positive or negative IVF outcome was added during the in-vitro maturation of bovine oocytes. Negative and obese follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool. |
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Language
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English
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Source (journal)
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Reproductive biomedicine online. - Cambridge
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Publication
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Cambridge
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2015
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ISSN
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1472-6483
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DOI
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10.1016/J.RBMO.2014.10.008
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Volume/pages
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30
:2
(2015)
, p. 203-207
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ISI
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000349087000015
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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