Publication
Title
A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV genotyping extra assay for the detection and typing of human papillomavirus
Author
Abstract
A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler® 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.
Language
English
Source (journal)
Methods in molecular biology. - Totowa, N.J., 1984, currens
Publication
Totowa, N.J. : 2015
ISSN
1064-3745 [print]
1940-6029 [online]
Volume/pages
1249(2015), p. 27-35
Full text (Publishers DOI)
Full text (publishers version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identification
Creation 07.04.2015
Last edited 14.10.2015
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