Title
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A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV genotyping extra assay for the detection and typing of human papillomavirus
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Author
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Abstract
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A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler® 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay. |
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Language
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English
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Source (journal)
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Methods in molecular biology. - Totowa, N.J., 1984, currens
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Publication
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Totowa, N.J.
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2015
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ISSN
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1064-3745
[print]
1940-6029
[online]
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DOI
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10.1007/978-1-4939-2013-6_2
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Volume/pages
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1249
(2015)
, p. 27-35
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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