|
Title
|
|
|
|
A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV genotyping extra assay for the detection and typing of human papillomavirus
|
|
|
Author
|
|
|
|
|
|
|
Abstract
|
|
|
|
| A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler® 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay. |
|
|
|
Language
|
|
|
|
English
|
|
|
Source (journal)
|
|
|
|
Methods in molecular biology. - Totowa, N.J., 1984, currens
|
|
|
Publication
|
|
|
|
Totowa, N.J.
:
2015
|
|
|
ISSN
|
|
|
|
1064-3745
[print]
1940-6029
[online]
|
|
|
DOI
|
|
|
|
10.1007/978-1-4939-2013-6_2
|
|
|
Volume/pages
|
|
|
|
1249
(2015)
, p. 27-35
|
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|
|
Full text (publisher's version - intranet only)
|
|
|
|
|
|