Publication
Title
Bone matrix vesicle-bound alkaline phosphatase for the assessment of peripheral blood admixture to human bone marrow aspirates
Author
Abstract
Purpose Peripheral blood (PB) admixture should be minimized during numerical and functional, as well as cytokinetic analysis of bone marrow (BM) aspirates for research purposes. Therefore, purity assessment of the BM aspirate should be performed in advance. We investigated whether bone matrix vesicle (BMV)-bound bone alkaline phosphatase (ALP) could serve as a marker for the purity of BM aspirates. Results Total ALP activity was significantly higher in BM serum (97 (176124) U/L, median (range)) compared to PB serum (63 (5273) U/L, p < 0.001). Agarose gel electrophoresis showed a unique bone ALP fraction in BM, which was absent in PB. Native polyacrylamide gel electrophoresis revealed the high molecular weight of this fraction, corresponding with membrane-bound ALP from bone matrix vesicles (BMV), as evidenced by electron microscopy. A serial PB admixture experiment of bone cylinder supernatant samples, rich in BMV-bound ALP, confirmed the sensitivity of this proposed quality assessment method. Furthermore, a BMV ALP fraction of ≥ 15% is suggested as cut-off value for minimal BM quality. Moreover, the BM purity declines rapidly with larger aspirated BM volumes. Conclusion The exclusive presence of BMV-bound ALP in BM could serve as a novel marker to assess purity of BM aspirates.
Language
English
Source (journal)
Clinica chimica acta. - Amsterdam
Publication
Amsterdam : 2015
ISSN
0009-8981
Volume/pages
446(2015), p. 253-260
ISI
000356745600045
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
[E?say:metaLocaldata.cgzprojectinf]
The extent of bone marrow dysfunction and the potential to reverse underlying mechanisms in patients with ischemic cardiomyopathy.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 20.05.2015
Last edited 20.10.2017
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