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Title
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Evaluation of random sequence and repetitive motif primed polymerase chain reaction typing of **Helicobacter pylori**
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Author
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Abstract
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| The suitability of PCR based DNA fingerprinting of H. pylori strains using random sequences or fragments of repetitive elements as primers was evaluated. In contrast with most other DNA-based fingerprinting techniques used in H. pylori epidemiology, this is a rapid and technically simple method allowing to study the distribution of a single type in different individuals. A disadvantage is that clonal variants or subtypes of strains may not be recognized if only a single primer is applied. It is strongly recommended to evaluate DNA amplification patterns obtained with a least two different primers for the determination of relationships between isolates. Ideally, a second typing technique should be used as well. |
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Language
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English
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Source (journal)
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Systematic and applied microbiology. - Jena, 1983, currens
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Publication
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Jena
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Elsevier
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1995
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ISSN
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0723-2020
[print]
1618-0984
[online]
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DOI
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10.1016/S0723-2020(11)80428-5
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Volume/pages
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18
(1995)
, p. 357-362
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ISI
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A1995TL82000007
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Full text (Publisher's DOI)
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