Title
Evaluation of random sequence and repetitive motif primed polymerase chain reaction typing of **Helicobacter pylori** Evaluation of random sequence and repetitive motif primed polymerase chain reaction typing of **Helicobacter pylori**
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Stuttgart ,
Subject
Biology
Engineering sciences. Technology
Source (journal)
Systematic and applied microbiology. - Stuttgart
Volume/pages
18(1995) , p. 357-362
ISSN
0723-2020
ISI
A1995TL82000007
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
The suitability of PCR based DNA fingerprinting of H. pylori strains using random sequences or fragments of repetitive elements as primers was evaluated. In contrast with most other DNA-based fingerprinting techniques used in H. pylori epidemiology, this is a rapid and technically simple method allowing to study the distribution of a single type in different individuals. A disadvantage is that clonal variants or subtypes of strains may not be recognized if only a single primer is applied. It is strongly recommended to evaluate DNA amplification patterns obtained with a least two different primers for the determination of relationships between isolates. Ideally, a second typing technique should be used as well.
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