Publication
Title
Characterization of top-down ETD in a travelling-wave ion guide
Author
Abstract
Top-down sequencing methods are becoming increasingly relevant for protein characterization, in particular electron capture (ECD) and electron transfer dissociation (ETD) which allow for extensive backbone cleavage with minimal side reactions. The ability to obtain sequence-specific fragments while maintaining aspects of the higher-order structure, as well as the position of deuterium labels in H/D exchange, has attracted interest from scientists in the field of structural proteomics. Recently, ETD has also been combined with ion mobility on commercially available quadrupole/time-of-flight instruments, and this implementation paves the way to novel structural studies and investigation of the ETD process itself. In the current work, we investigate the use of ETD for fragmentation of standard peptides and proteins and provide a detailed description of the effect of the parameters controlling the time and efficiency of the reaction. We also highlight how the combination with ion mobility separation after electron transfer provides extended analytical benefits, such as assignment of fragments to a specific charge-reduced state of the precursor.
Language
English
Source (journal)
Methods: a companion to methods in enzymology. - San Diego, Calif.
Publication
San Diego, Calif. : 2015
ISSN
1046-2023
DOI
10.1016/J.YMETH.2015.05.019
Volume/pages
89 (2015) , p. 22-29
ISI
000365062800004
Pubmed ID
26014039
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 19.06.2015
Last edited 09.10.2023
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