Title
VEGF-targeted magnetic nanoparticles for MRI visualization of brain tumor VEGF-targeted magnetic nanoparticles for MRI visualization of brain tumor
Author
Faculty/Department
Faculty of Sciences. Physics
Publication type
article
Publication
,
Subject
Human medicine
Engineering sciences. Technology
Source (journal)
Nanomedicine: nanotechnology, biology and medicine. - Place of publication unknown
Volume/pages
11(2015) :4 , p. 825-833
ISSN
1549-9634
ISI
000354559600004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
This work is focused on synthesis and characterization of targeted magnetic nanoparticles as magnetic resonance imaging (MRI) agents for in vivo visualization of gliomas. Ferric oxide (Fe3O4) cores were synthesized by thermal decomposition and coated with bovine serum albumin (BSA) to form nanoparticles with D-eff of 53 +/- 9 nm. The BSA was further cross-linked to improve colloidal stability. Monoclonal antibodies against vascular endothelial growth factor (mAbVEGF) were covalently conjugated to BSA through a polyethyleneglycol linker. Here we demonstrate that 1) BSA coated nanoparticles are stable and non-toxic to different cells at concentration up to 2.5 mg/mL; 2) conjugation of monoclonal antibodies to nanoparticles promotes their binding to VEGF-positive glioma C6 cells in vitro; 3) targeted nanoparticles are effective in MRI visualization of the intracranial glioma. Thus, mAbVEGF-targeted BSA-coated magnetic nanoparticles are promising MRI contrast agents for glioma visualization. (C) 2015 Elsevier Inc. All rights reserved.
E-info
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https://repository.uantwerpen.be/docman/iruaauth/1298ec/b4d126351.pdf
Full text (open access)
https://repository.uantwerpen.be/docman/irua/3ad2d2/126351.pdf
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