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Title
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Microfluidic system for the identification of bacterial pathogens causing urinary tract infections
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Author
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Abstract
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| Urinary tract infections (UTIs) are among the most common bacterial infections and pose a significant healthcare burden. The growing trend in antibiotic resistance makes it mandatory to develop diagnostic kits which allow not only the determination of a pathogen but also the antibiotic resistances. We have developed a microfluidic cartridge which takes a direct urine sample, extracts the DNA, performs an amplification using batch-PCR and flows the sample over a microarray which is printed into a microchannel for fluorescence detection. The cartridge is injection-molded out of COP and contains a set of two-component injection-molded rotary valves to switch between input and to isolate the PCR chamber during thermocycling. The hybridization probes were spotted directly onto a functionalized section of the outlet microchannel. We have been able to successfully perform PCR of E. coli in urine in this chip and perform a fluorescence detection of PCR products. An upgraded design of the cartridge contains the buffers and reagents in blisters stored on the chip. |
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Language
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English
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Source (journal)
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Proceedings of the Society of Photo-optical Instrumentation Engineers / SPIE: International Society for Optical Engineering. - Bellingham, Wash.
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Source (book)
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Conference on Microfluidics, BioMEMS, and Medical Microsystems XIII, FEB 07-09, 2015, San Francisco, CA
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Publication
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Bellingham
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Spie-int soc optical engineering
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2015
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ISSN
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0277-786X
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ISBN
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978-1-62841-410-3
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DOI
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10.1117/12.2077644
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Volume/pages
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9320
(2015)
, 5 p.
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Article Reference
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93200S
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ISI
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000354516300020
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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