Microfluidic system for the identification of bacterial pathogens causing urinary tract infections
van der Wijngaart, Wouter
Faculty of Medicine and Health Sciences
Bellingham :Spie-int soc optical engineering
MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS XIII
Conference on Microfluidics, BioMEMS, and Medical Microsystems XIII, FEB 07-09, 2015, San Francisco, CA
, 5 p.
University of Antwerp
Urinary tract infections (UTIs) are among the most common bacterial infections and pose a significant healthcare burden. The growing trend in antibiotic resistance makes it mandatory to develop diagnostic kits which allow not only the determination of a pathogen but also the antibiotic resistances. We have developed a microfluidic cartridge which takes a direct urine sample, extracts the DNA, performs an amplification using batch-PCR and flows the sample over a microarray which is printed into a microchannel for fluorescence detection. The cartridge is injection-molded out of COP and contains a set of two-component injection-molded rotary valves to switch between input and to isolate the PCR chamber during thermocycling. The hybridization probes were spotted directly onto a functionalized section of the outlet microchannel. We have been able to successfully perform PCR of E. coli in urine in this chip and perform a fluorescence detection of PCR products. An upgraded design of the cartridge contains the buffers and reagents in blisters stored on the chip.