Microfluidic system for the identification of bacterial pathogens causing urinary tract infectionsMicrofluidic system for the identification of bacterial pathogens causing urinary tract infections
van der Wijngaart, Wouter
Faculty of Medicine and Health Sciences
Vaccine & Infectious Disease Institute (VAXINFECTIO)
2015Bellingham :Spie-int soc optical engineering, 2015
MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS XIII
Conference on Microfluidics, BioMEMS, and Medical Microsystems XIII, FEB 07-09, 2015, San Francisco, CA
9320(2015), 5 p.
University of Antwerp
Urinary tract infections (UTIs) are among the most common bacterial infections and pose a significant healthcare burden. The growing trend in antibiotic resistance makes it mandatory to develop diagnostic kits which allow not only the determination of a pathogen but also the antibiotic resistances. We have developed a microfluidic cartridge which takes a direct urine sample, extracts the DNA, performs an amplification using batch-PCR and flows the sample over a microarray which is printed into a microchannel for fluorescence detection. The cartridge is injection-molded out of COP and contains a set of two-component injection-molded rotary valves to switch between input and to isolate the PCR chamber during thermocycling. The hybridization probes were spotted directly onto a functionalized section of the outlet microchannel. We have been able to successfully perform PCR of E. coli in urine in this chip and perform a fluorescence detection of PCR products. An upgraded design of the cartridge contains the buffers and reagents in blisters stored on the chip.