Title
Microfluidic system for the identification of bacterial pathogens causing urinary tract infections Microfluidic system for the identification of bacterial pathogens causing urinary tract infections
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
conferenceObject
Publication
Bellingham :Spie-int soc optical engineering ,
Subject
Physics
Source (journal)
MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS XIII
Source (book)
Conference on Microfluidics, BioMEMS, and Medical Microsystems XIII, FEB 07-09, 2015, San Francisco, CA
Volume/pages
9320(2015) , 5 p.
ISSN
0277-786X
Article Reference
93200S
ISBN
978-1-62841-410-3
ISI
000354516300020
Carrier
E-only publicatie
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Urinary tract infections (UTIs) are among the most common bacterial infections and pose a significant healthcare burden. The growing trend in antibiotic resistance makes it mandatory to develop diagnostic kits which allow not only the determination of a pathogen but also the antibiotic resistances. We have developed a microfluidic cartridge which takes a direct urine sample, extracts the DNA, performs an amplification using batch-PCR and flows the sample over a microarray which is printed into a microchannel for fluorescence detection. The cartridge is injection-molded out of COP and contains a set of two-component injection-molded rotary valves to switch between input and to isolate the PCR chamber during thermocycling. The hybridization probes were spotted directly onto a functionalized section of the outlet microchannel. We have been able to successfully perform PCR of E. coli in urine in this chip and perform a fluorescence detection of PCR products. An upgraded design of the cartridge contains the buffers and reagents in blisters stored on the chip.
E-info
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