Title
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Intercalation of proflavine in ssDNA aptamers : effect on binding of the specific target chloramphenicol
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Author
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Abstract
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The structural modification of ssDNA-based aptamers upon specific binding of its target molecule leads to changes of the charge-transfer resistance (Rct) of a negatively-charged free-diffusing redox probe. The aptamer adopts a structure due to self-hybridization which is stabilized using profalvine as intercalator. The pre-organized aptamer structure is used to detect chloramphenicol (CAP) requiring a substantial change of the aptamer structure indicated by a CAP concentration dependent increase in the Rct values. Pre-incubation of the aptamer-modified electrode with an intercalator allows for the modulation of the aptamer/target interaction and hence for a modulation of the CAP-dependent variation of the Rct values. |
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Language
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English
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Source (journal)
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Electroanalysis. - Deerfield Beach, Fla
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Publication
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Deerfield Beach, Fla
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2015
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ISSN
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1040-0397
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DOI
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10.1002/ELAN.201500192
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Volume/pages
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27
:8
(2015)
, p. 1836-1841
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ISI
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000359737500006
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Full text (Publisher's DOI)
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