Title
Intercalation of proflavine in ssDNA aptamers : effect on binding of the specific target chloramphenicolIntercalation of proflavine in ssDNA aptamers : effect on binding of the specific target chloramphenicol
Author
Faculty/Department
Faculty of Sciences. Chemistry
Research group
AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Publication type
article
Publication
Deerfield Beach, Fla,
Subject
Physics
Chemistry
Source (journal)
Electroanalysis. - Deerfield Beach, Fla
Volume/pages
27(2015):8, p. 1836-1841
ISSN
1040-0397
ISI
000359737500006
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The structural modification of ssDNA-based aptamers upon specific binding of its target molecule leads to changes of the charge-transfer resistance (Rct) of a negatively-charged free-diffusing redox probe. The aptamer adopts a structure due to self-hybridization which is stabilized using profalvine as intercalator. The pre-organized aptamer structure is used to detect chloramphenicol (CAP) requiring a substantial change of the aptamer structure indicated by a CAP concentration dependent increase in the Rct values. Pre-incubation of the aptamer-modified electrode with an intercalator allows for the modulation of the aptamer/target interaction and hence for a modulation of the CAP-dependent variation of the Rct values.
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