Publication
Title
Intercalation of proflavine in ssDNA aptamers : effect on binding of the specific target chloramphenicol
Author
Abstract
The structural modification of ssDNA-based aptamers upon specific binding of its target molecule leads to changes of the charge-transfer resistance (Rct) of a negatively-charged free-diffusing redox probe. The aptamer adopts a structure due to self-hybridization which is stabilized using profalvine as intercalator. The pre-organized aptamer structure is used to detect chloramphenicol (CAP) requiring a substantial change of the aptamer structure indicated by a CAP concentration dependent increase in the Rct values. Pre-incubation of the aptamer-modified electrode with an intercalator allows for the modulation of the aptamer/target interaction and hence for a modulation of the CAP-dependent variation of the Rct values.
Language
English
Source (journal)
Electroanalysis. - Deerfield Beach, Fla
Publication
Deerfield Beach, Fla : 2015
ISSN
1040-0397
Volume/pages
27:8(2015), p. 1836-1841
ISI
000359737500006
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 09.07.2015
Last edited 08.11.2017
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