Title
The cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activityThe cytotoxicity of elderberry ribosome-inactivating proteins is not solely determined by their protein translation inhibition activity
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Research group
Laboratory of cell biology and histology
Publication type
article
Publication
Subject
Veterinary medicine
Source (journal)
PLoS ONE
Volume/pages
10(2015):7, p. 1-23
ISSN
1932-6203
Article Reference
e0132389
Carrier
E-only publicatie
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Although the protein translation inhibition activity of ribosome inactivating proteins (RIPs) is well documented, little is known about the contribution of the lectin chain to the biological activity of these proteins. In this study, we compared the in vitro and intracellular activity of several S. nigra (elderberry) RIPs and non-RIP lectins. Our data demonstrate that RIPs from elderberry are much more toxic to HeLa cells than to primary fibroblasts. Differences in the cytotoxicity between the elderberry proteins correlated with differences in glycan specificity of their lectin domain, cellular uptake efficiency and intracellular destination. Despite the fact that the bulk of the RIPs accumulated in the lysosomes and partly in the Golgi apparatus, we could demonstrate effective inhibition of protein synthesis in cellula. As we also observed cytotoxicity for non-RIP lectins, it is clear that the lectin chain triggers additional pathways heralding cell death. Our data suggest that one of these pathways involves the induction of autophagy.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/f1130e/126534.pdf
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