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Title
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Incubation at 32.5°C and above causes malformations in the zebrafish embryo
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Author
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Abstract
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| Zebrafish embryos are increasingly used for developmental toxicity screening of candidate drugs and are occasionally co-incubated with a metabolic activation system at 32 °C for 1, 2 or 4 h, depending on their developmental stage. As this temperature is higher than the optimal temperature for zebrafish embryonic development (2628.5 °C), we investigated whether continuous incubation of zebrafish embryos from 2.5 until 96 h post fertilization (hpf) at high temperatures (30.536.5 °C) causes malformations. At 32.5 °C tail malformations were observed as early as 24 hpf, and these became even more prominent at 34.5 and 36.5 °C. Cardiovascular and head malformations, edema and blood accumulations throughout the body were present at 36.5 °C. Finally, temperatures higher than 28.5 °C accelerated embryonic development except for 36.5 °C, at which a lower hatching rate and hatching enzyme activity were observed. In conclusion, incubation of zebrafish embryos at 32.5 °C and above from 2.5 until 96 hpf causes malformations as early as 24 hpf. |
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Language
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English
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Source (journal)
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Reproductive toxicology. - Elmsford, N.Y.
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Publication
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Elmsford, N.Y.
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2015
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ISSN
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0890-6238
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DOI
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10.1016/J.REPROTOX.2015.05.006
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Volume/pages
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56
(2015)
, p. 56-63
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ISI
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000359183400006
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Pubmed ID
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26005098
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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