Title
Incubation at 32.5°C and above causes malformations in the zebrafish embryo
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Veterinary Sciences
Publication type
article
Publication
Elmsford, N.Y. ,
Subject
Biology
Pharmacology. Therapy
Veterinary medicine
Source (journal)
Reproductive toxicology. - Elmsford, N.Y.
Volume/pages
56(2015) , p. 56-63
ISSN
0890-6238
ISI
000359183400006
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Zebrafish embryos are increasingly used for developmental toxicity screening of candidate drugs and are occasionally co-incubated with a metabolic activation system at 32 °C for 1, 2 or 4 h, depending on their developmental stage. As this temperature is higher than the optimal temperature for zebrafish embryonic development (2628.5 °C), we investigated whether continuous incubation of zebrafish embryos from 2.5 until 96 h post fertilization (hpf) at high temperatures (30.536.5 °C) causes malformations. At 32.5 °C tail malformations were observed as early as 24 hpf, and these became even more prominent at 34.5 and 36.5 °C. Cardiovascular and head malformations, edema and blood accumulations throughout the body were present at 36.5 °C. Finally, temperatures higher than 28.5 °C accelerated embryonic development except for 36.5 °C, at which a lower hatching rate and hatching enzyme activity were observed. In conclusion, incubation of zebrafish embryos at 32.5 °C and above from 2.5 until 96 hpf causes malformations as early as 24 hpf.
E-info
https://repository.uantwerpen.be/docman/iruaauth/d467a4/d05c0dfc553.pdf
Full text (open access)
https://repository.uantwerpen.be/docman/irua/40bc62/10512.pdf
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