Title
In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome In silico discovery of a nearly complete mitochondrial genome Numt in the dog (Canis lupus familiaris) nuclear genome
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
The Hague ,
Subject
Human medicine
Source (journal)
Genetica. - The Hague
Volume/pages
143(2015) :4 , p. 453-458
ISSN
0016-6707
ISI
000357044300007
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Through bacterial cloning, a non-specific product co-amplified in a previous whole mitochondrial genome study of Canis lupus familiaris was identified as part of a Numt on chromosome 29 of the dog. Even though further analysis confirmed the fidelity of the mitochondrial genome sequencing results, it still highlighted the risk of Numt contamination. A computer-based search of the dog's nuclear genome for segments homologous to the mtDNA sequence revealed the extent of this risk. Over 150 Numts of various sizes were observed throughout all but two chromosomes, covering all positions of the mtDNA. One of the Numts on chromosome 11 even covered over 95 % of the entire dog mtDNA sequence. This comprehensive list of Numts was provided to assist researchers with the evaluation of dog mtDNA sequencing protocols for Numt co-amplification.
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