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Title
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Potentiometric detection in UPLC as an easy alternative to determine cocaine in biological samples
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Author
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Abstract
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| The analytical methods which are often used for the determination of cocaine in complex biological matrices are a prescreening immunoassay and confirmation by chromatography combined with mass spectrometry. We suggest an ultra-high-pressure liquid chromatography combined with a potentiometric detector, as a fast and practical method to detect and quantify cocaine in biological samples. An adsorption/desorption model was used to investigate the usefulness of the potentiometric detector to determine cocaine in complex matrices. Detection limits of 6.3ngmL(-1) were obtained in plasma and urine, which is below the maximum residue limit (MRL) of 25ngmL(-1). A set of seven plasma samples and 10 urine samples were classified identically by both methods as exceeding the MRL or being inferior to it. The results obtained with the UPLC/potentiometric detection method were compared with the results obtained with the UPLC/MS method for samples spiked with varying cocaine concentrations. The intraclass correlation coefficient was 0.997 for serum (n =7) and 0.977 for urine (n =8). As liquid chromatography is an established technique, and as potentiometry is very simple and cost-effective in terms of equipment, we believe that this method is potentially easy, inexpensive, fast and reliable. Copyright (c) 2014 John Wiley & Sons, Ltd. |
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Language
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English
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Source (journal)
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Biomedical chromatography. - London
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Publication
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London
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2015
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ISSN
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0269-3879
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DOI
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10.1002/BMC.3400
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Volume/pages
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29
:7
(2015)
, p. 1124-1129
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ISI
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000356694000020
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Pubmed ID
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25516367
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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