Title
Monitoring the laccase reaction of vanillin and poplar hydrolysate Monitoring the laccase reaction of vanillin and poplar hydrolysate
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Faculty of Applied Engineering Sciences
Publication type
article
Publication
Oxford ,
Subject
Chemistry
Biology
Engineering sciences. Technology
Source (journal)
Journal of chemical technology and biotechnology / Society of Chemical Industry [London] - Oxford, 1986, currens
Volume/pages
91(2016) :6 , p. 1914-1922
ISSN
0268-2575
ISI
000375768300040
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
BACKGROUND Laccase is an intensively researched enzyme for industrial use. Except for decolorisation measurements, HPLC analysis is the conventional method for monitoring the phenolic removal during laccase enzyme reaction. This paper reports an investigation of the continuous UV absorbance follow-up of the laccase reaction with steam pretreated poplar hydrolysate. RESULTS Vanillin was used as a model substrate and lignocellulose xylose rich fraction (XRF) as a biologically complex substrate for laccase detoxification. The reaction was followed by HPLC-UV as well as by UV spectrometric measurements. Results suggest that the reaction can be successfully monitored by measuring the change of UV absorbance at 280 nm, without previous compound separation. In case of XRF experiments the spectrophotometric follow-up is especially useful, as HPLC analysis takes a long time and provides less information than in case of single substrates. The method seems to be suitable for optimization and process control. CONCLUSION The obtained results can help to construct a fast, easy and straightforward monitoring system for laccase-phenolic substrate reactions.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/e767b0/75f8c53d.pdf
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