Publication
Title
Monitoring the laccase reaction of vanillin and poplar hydrolysate
Author
Abstract
BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for decolorization measurements, high performance liquid chromatography (HPLC) analysis is the conventional method for monitoring the phenolic removal during laccase enzyme reaction. This paper reports an investigation of the continuous UV absorbance follow-up of the laccase reaction with steam pretreated poplar hydrolysate. RESULTS: Vanillin was used as a model substrate and lignocellulose xylose rich fraction (XRF) as a biologically complex substrate for laccase detoxification. The reaction was followed by HPLC-UV as well as by UV spectrometric measurements. Results suggest that the reaction can be successfully monitored by measuring the change of UV absorbance at 280 nm, without previous compound separation. In the case of XRF experiments the spectrophotometric follow-up is especially useful, as HPLC analysis takes a long time and provides less information than in the case of single substrates. The method seems to be suitable for optimization and process control. CONCLUSION: The results obtained can help to construct a fast, easy and straightforward monitoring system for laccase-phenolic substrate reactions. (C) 2015 Society of Chemical Industry
Language
English
Source (journal)
Journal of chemical technology and biotechnology / Society of Chemical Industry [London] - Oxford, 1986, currens
Publication
Oxford : 2016
ISSN
0268-2575 [print]
1097-4660 [online]
DOI
10.1002/JCTB.4789
Volume/pages
91 :6 (2016) , p. 1914-1922
ISI
000375768300040
Full text (Publisher's DOI)
Full text (open access)
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UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
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Creation 01.10.2015
Last edited 09.10.2023
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