Title
Extending native mass spectrometry approaches to integral membrane proteins Extending native mass spectrometry approaches to integral membrane proteins
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
Berlin :Walter de gruyter gmbh ,
Subject
Chemistry
Biology
Source (journal)
Biological chemistry. - Berlin
Volume/pages
396(2015) :9-10 , p. 991-1002
ISSN
1431-6730
ISI
000359110800005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Recent developments in native mass spectrometry and ion mobility have made it possible to analyze the composition and structure of membrane protein complexes in the gas-phase. In this short review we discuss the experimental strategies that allow to elucidate aspects of the dynamic structure of these important drug targets, such as the structural effects of lipid binding or detection of co-populated conformational and assembly states during gating on an ion channel. As native mass spectrometry relies on nano-electrospray of natively reconstituted proteins, a number of commonly used lipid-and detergentbased reconstitution systems have been evaluated for their compatibility with this approach, and parameters for the release of intact, native-like folded membrane proteins studied in the gas-phase. The strategy thus developed can be employed for the investigation of the subunit composition and stoichiometry, oligomeric state, conformational changes, and lipid and drug binding of integral membrane proteins.
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Full text (open access)
https://repository.uantwerpen.be/docman/irua/33e7b4/127747.pdf
Handle