Title
Characterization of the newly isolated lytic bacteriophages KTN6 and KT28 and their efficacy against **Pseudomonas aeruginosa** biofilm Characterization of the newly isolated lytic bacteriophages KTN6 and KT28 and their efficacy against **Pseudomonas aeruginosa** biofilm
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Subject
Biology
Engineering sciences. Technology
Source (journal)
PLoS ONE
Volume/pages
10(2015) :5 , p. 1-20
ISSN
1932-6203
Article Reference
e0127603
Carrier
E-only publicatie
Target language
English (eng)
Full text (Publishers DOI)
Abstract
We here describe two novel lytic phages, KT28 and KTN6, infecting Pseudomonas aeruginosa, isolated from a sewage sample from an irrigated field near Wroclaw, in Poland. Both viruses show characteristic features of Pbunalikevirus genus within the Myoviridae family with respect to shape and size of head/tail, as well as LPS host receptor recognition. Genome analysis confirmed the similarity to other PB1-related phages, ranging between 48 and 96%. Pseudomonas phage KT28 has a genome size of 66,381 bp and KTN6 of 65,994 bp. The latent period, burst size, stability and host range was determined for both viruses under standard laboratory conditions. Biofilm eradication efficacy was tested on peg-lid plate assay and PET membrane surface. Significant reduction of colony forming units was observed (70-90%) in 24 h to 72 h old Pseudomonas aeruginosa PAO1 biofilm cultures for both phages. Furthermore, a pyocyanin and pyoverdin reduction tests reveal that tested phages lowers the amount of both secreted dyes in 48-72 h old biofilms. Diffusion and goniometry experiments revealed the increase of diffusion rate through the biofilm matrix after phage application. These characteristics indicate these phages could be used to prevent Pseudomonas aeruginosa infections and biofilm formation. It was also shown, that PB1-related phage treatment of biofilm caused the emergence of stable phage-resistant mutants growing as small colony variants.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/fecc65/128248.pdf
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