Publication
Title
Instability of bacteriophages in spray-dried trehalose powders is caused by crystallization of the matrix
Author
Abstract
Spray drying is a valuable technique in pharmaceutical dosage formulation, capable of producing amorphous, spherical powders, suitable for pulmonary deposition and further downstream processing. In this study, we show that spray drying bacteriophages together with trehalose results in an amorphous powder matrix with high glass transition temperature (between 116 and 118 °C), typical for amorphous trehalose. These powders are stable at low temperatures (4 °C) and relative humidity (0%). However, high humidity causes crystallization of the amorphous matrix, destroying the embedded phages. Furthermore, storage at higher temperature (25 °C) causes thermal instability of the embedded phages. The results show that storage conditions are important parameters to take into account in phage therapy development. The resulting particles are hollow spheres, with suitable aerodynamic diameters for deposition into the deep lungs. This opens possibilities to use these phage-containing powder formulations to tackle pulmonary infectious diseases, especially caused by antibiotic resistant pathogens.
Language
English
Source (journal)
International journal of pharmaceutics. - Amsterdam
Publication
Amsterdam : 2014
ISSN
0378-5173
Volume/pages
472:1/2(2014), p. 202-205
ISI
000340328400024
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 19.10.2015
Last edited 11.11.2017