Title
Instability of bacteriophages in spray-dried trehalose powders is caused by crystallization of the matrix Instability of bacteriophages in spray-dried trehalose powders is caused by crystallization of the matrix
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Amsterdam ,
Subject
Pharmacology. Therapy
Engineering sciences. Technology
Source (journal)
International journal of pharmaceutics. - Amsterdam
Volume/pages
472(2014) :1/2 , p. 202-205
ISSN
0378-5173
ISI
000340328400024
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Spray drying is a valuable technique in pharmaceutical dosage formulation, capable of producing amorphous, spherical powders, suitable for pulmonary deposition and further downstream processing. In this study, we show that spray drying bacteriophages together with trehalose results in an amorphous powder matrix with high glass transition temperature (between 116 and 118 °C), typical for amorphous trehalose. These powders are stable at low temperatures (4 °C) and relative humidity (0%). However, high humidity causes crystallization of the amorphous matrix, destroying the embedded phages. Furthermore, storage at higher temperature (25 °C) causes thermal instability of the embedded phages. The results show that storage conditions are important parameters to take into account in phage therapy development. The resulting particles are hollow spheres, with suitable aerodynamic diameters for deposition into the deep lungs. This opens possibilities to use these phage-containing powder formulations to tackle pulmonary infectious diseases, especially caused by antibiotic resistant pathogens.
E-info
https://repository.uantwerpen.be/docman/iruaauth/37a8a8/128250.pdf
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