Title
CIM® monolithic anion-exchange chromatography as a useful alternative to CsCl gradient purification of bacteriophage particles CIM® monolithic anion-exchange chromatography as a useful alternative to CsCl gradient purification of bacteriophage particles
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
New York ,
Subject
Biology
Pharmacology. Therapy
Engineering sciences. Technology
Source (journal)
Virology. - New York
Volume/pages
434(2012) :2 , p. 264-270
ISSN
0042-6822
ISI
000312509300016
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The use of anion-exchange chromatography was investigated as an alternative method to concentrate and purify bacterial viruses, and parameters for different bacteriophages were compared. Chromatography was performed with Convective Interactive Media® monoliths, with three different volumes and two matrix chemistries. Eleven morphologically distinct phages were tested, infecting five different bacterial species. For each of the phages tested, a protocol was optimized, including the choice of column chemistry, loading, buffer and elution conditions. The capacity and recovery of the phages on the columns varied considerably between phages. We conclude that anion-exchange chromatography with monoliths is a valid alternative to the more traditional CsCl purification, has upscaling advantages, but it requires more extensive optimization.
E-info
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