Publication
Title
CIM® monolithic anion-exchange chromatography as a useful alternative to CsCl gradient purification of bacteriophage particles
Author
Abstract
The use of anion-exchange chromatography was investigated as an alternative method to concentrate and purify bacterial viruses, and parameters for different bacteriophages were compared. Chromatography was performed with Convective Interactive Media® monoliths, with three different volumes and two matrix chemistries. Eleven morphologically distinct phages were tested, infecting five different bacterial species. For each of the phages tested, a protocol was optimized, including the choice of column chemistry, loading, buffer and elution conditions. The capacity and recovery of the phages on the columns varied considerably between phages. We conclude that anion-exchange chromatography with monoliths is a valid alternative to the more traditional CsCl purification, has upscaling advantages, but it requires more extensive optimization.
Language
English
Source (journal)
Virology. - New York
Publication
New York : 2012
ISSN
0042-6822
Volume/pages
434:2(2012), p. 264-270
ISI
000312509300016
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 19.10.2015
Last edited 11.11.2017