Effects of aerobic interval training and continuous training on cellular markers of endothelial integrity in coronary artery disease : a SAINTEX-CAD substudy
van Craenenbroeck, Emeline M.
Van Craenenbroeck, Amaryllis H.
Faculty of Medicine and Health Sciences
American journal of physiology: heart and circulatory physiology. - Bethesda, Md
, p. H1876-H1882
University of Antwerp
Background: In this large multicenter trial, we aimed to assess the effect of aerobic exercise training in stable coronary artery disease (CAD) patients on cellular markers of endothelial integrity, and examine their relation with improvement of endothelial function. Methods: Two-hundred CAD patients (LVEF >40%, 90% male, mean age 58.4±9.1 years) were randomized on a 1:1 base to a supervised 12-week rehabilitation program of either aerobic interval training (AIT) or aerobic continuous training (ACT) on a bicycle. At baseline and after 12 weeks, numbers of circulating CD34+/KDR+/CD45dim endothelial progenitor cells (EPC), CD31+/CD3+/CXCR4+ angiogenic T-cells and CD31+/CD42b- endothelial microparticles (EMP) were analyzed by flow cytometry. Endothelial function was assessed by flow-mediated dilation (FMD) of the brachial artery. Results: After 12 weeks of AIT or ACT, numbers of circulating EPC, angiogenic T-cells and EMP were comparable to baseline levels. Whereas improvement in peak VO2 was correlated to improvement in FMD (pearson r = 0.17, p = 0.035), a direct correlation of baseline or post-training EPC, angiogenic T-cells and EMP levels with FMD was absent. Baseline EMP related inversely to the magnitude of the increases in peak VO2 (spearman rho = -0.245, p = 0.027) and FMD (spearman rho = -0.374, p = 0.001) following exercise training. Conclusions. Endothelial function improvement in response to exercise training in CAD patients did not relate to altered levels of EPC and angiogenic T-cells and/or a diminished shedding of EMP into the circulation. EMP flow cytometry may be predictive of the increase in aerobic capacity and endothelial function.