Title
Role of both actin-myosin cross bridges and NO-cGMP pathway modulators in the contraction and relaxation of human placental stem villi Role of both actin-myosin cross bridges and NO-cGMP pathway modulators in the contraction and relaxation of human placental stem villi
Author
Publication type
article
Publication
Boston, Mass. ,
Subject
Biology
Human medicine
Source (journal)
Placenta. - Boston, Mass.
Volume/pages
34(2013) :12 , p. 1163-1169
ISSN
0143-4004
ISI
000328309000007
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Introduction: Human placental stem villi (PSV) present contractile properties. We studied the role of actin-myosin cross bridges (CBs) and the effects of NO-cGMP pathway modulators in the PSV contraction and relaxation. Methods: In vitro contractile properties were investigated in 71 PSV from term human placentas studied according to their long axis. Contraction was induced by both KCl and electrical tetanic stimulation. Relaxation was induced by inhibiting the CB cycle with either 2,3-butanedione monoxime (BDM) or blebbistatin (BLE) and by activating the NO-cGMP pathway with isosorbide dinitrate (ISDN), sildenafil (SIL) or ISDN + SIL. Results: PSV tension slowly increased by 140% of the basal tone after KCl exposure and by 85% after tetanus. The addition of BDM, BLE, ISDN, SIL and ISDN + SIL induced a relaxation of PSV, the overall time course of relaxation (in s) was respectively (means +/- SD) 3412 +/- 1904, 14,250 +/- 3095*, 3813 +/- 1383, 2883 +/- 1188 and 2440 +/- 477; significantly longer in BLE compared with BDM, ISDN, SIL and ISDN + SIL:*p <0.001). the overall time course of relaxation (in s) was respectively (means +/- SD) 3412 +/- 1904, 14,250 +/- 3095*, 3813 +/- 1383, 2883 +/- 1188 and 2440 +/- 477; significantly longer in BLE compared with BDM, ISDN, SIL and ISDN + SIL:*p < 0.001). These relaxation kinetics were particularly slow. Other relaxation parametres, i.e., maximum lengthening, -peak dT/dt, and resting tension, did not differ between these 5 subgroups. Discussion and conclusion: Isolated human PSV were able to contract after both KCl exposure and tetanus. This increase in contractility was reversed by inhibiting the CB cycle with BDM or BLE and by stimulating the NO-cGMP pathway with ISDN or SIL. The association ISDN + SIL did not potentiate the relaxing processes (C) 2013 Elsevier Ltd. All rights reserved.
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