Title
Prelimbic cortical injections of a GABA agonist and antagonist : in vivo quantification of the effect in the rat brain using <tex>$[^{18}F]$</tex> FDG microPET Prelimbic cortical injections of a GABA agonist and antagonist : in vivo quantification of the effect in the rat brain using <tex>$[^{18}F]$</tex> FDG microPET
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
,
Subject
Human medicine
Computer. Automation
Source (journal)
Molecular imaging and biology. - Place of publication unknown
Volume/pages
17(2015) :6 , p. 856-864
ISSN
1536-1632
ISI
000364941700015
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Purpose We evaluated the glucose metabolism after microinjections of a GABAA antagonist, bicuculline, and a GABAA agonist, muscimol, in the rat prelimbic cortex (PL) by small animal positron emission tomography (μPET). Procedures Following a microinjection of either 0.5 μl bicuculline (0.1 mg/ml), muscimol (1 mg/ml), or saline in the left PL of 11 healthy male Sprague Dawley rats (250275 g), 2-deoxy-2-[18F]fluoro-β-d-glucose ([18F]FDG) PET images were acquired. Volume-of-interest (VOI)-based analysis and voxel-based statistical parametric mapping were performed (n = 9). Results VOI-based analysis revealed significantly different [18F]FDG uptake following bicuculline versus muscimol in PL (p < 0.001), infralimbic cortex (p < 0.01), and cingulate cortex (p < 0.01). Voxel-based analysis showed bicuculline induced widespread significant hypermetabolism throughout the brain while muscimol induced significant localized hypometabolism. Conclusions Here, we visualize functional GABAA-mediated correlations of the PL following pharmacological stimulation. This could serve as a reference and shed light on the working and focality of other stimulation paradigms targeting this region.
E-info
https://repository.uantwerpen.be/docman/iruaauth/9baac1/128991.pdf
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