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Title
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Prelimbic cortical injections of a GABA agonist and antagonist : in vivo quantification of the effect in the rat brain using FDG microPET
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Author
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Abstract
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| Purpose We evaluated the glucose metabolism after microinjections of a GABAA antagonist, bicuculline, and a GABAA agonist, muscimol, in the rat prelimbic cortex (PL) by small animal positron emission tomography (μPET). Procedures Following a microinjection of either 0.5 μl bicuculline (0.1 mg/ml), muscimol (1 mg/ml), or saline in the left PL of 11 healthy male Sprague Dawley rats (250275 g), 2-deoxy-2-[18F]fluoro-β-d-glucose ([18F]FDG) PET images were acquired. Volume-of-interest (VOI)-based analysis and voxel-based statistical parametric mapping were performed (n = 9). Results VOI-based analysis revealed significantly different [18F]FDG uptake following bicuculline versus muscimol in PL (p < 0.001), infralimbic cortex (p < 0.01), and cingulate cortex (p < 0.01). Voxel-based analysis showed bicuculline induced widespread significant hypermetabolism throughout the brain while muscimol induced significant localized hypometabolism. Conclusions Here, we visualize functional GABAA-mediated correlations of the PL following pharmacological stimulation. This could serve as a reference and shed light on the working and focality of other stimulation paradigms targeting this region. |
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Language
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English
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Source (journal)
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Molecular imaging and biology. - Place of publication unknown
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Publication
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Place of publication unknown
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2015
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ISSN
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1536-1632
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DOI
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10.1007/S11307-015-0859-Z
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Volume/pages
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17
:6
(2015)
, p. 856-864
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ISI
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000364941700015
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Pubmed ID
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25917751
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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