Publication
Title
Differences in the elemental isotope definition may lead to errors in modern mass-spectrometry-based proteomics
Author
Abstract
The elemental isotope definition used to calculate the theoretical masses and isotope distribution of (bio)molecules is considered to be a fixed, universal standard in mass-spectrometry-based proteomics. However, this is an incorrect assumption. In view of the ongoing advances in mass spectrometry technology, and in particular the ever-increasing mass precision, the elemental isotope definition and its variations should be taken into account. We illustrate the effect of the elemental isotope uncertainty on the theoretical and experimental masses with theoretical calculations and examples.
Language
English
Source (journal)
Analytical chemistry. - Washington, D.C., 1948, currens
Publication
Washington, D.C. : 2015
ISSN
0003-2700 [print]
5206-882X [online]
Volume/pages
87:21(2015), p. 10747-10754
ISI
000364354900017
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 09.12.2015
Last edited 21.07.2017
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