Title
Transverse sectioning of **Arabidopsis thaliana** leaves using resin embedding Transverse sectioning of **Arabidopsis thaliana** leaves using resin embedding
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Subject
Biology
Source (journal)
Bio-protocol
Volume/pages
5(2015) :18 , p. 1-5
ISSN
2331-8325
Article Reference
e1592
Carrier
E-only publicatie
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
The leaf is the major functional part of the shoot performing the bulk of photosynthetic activity. Its development is relatively plastic allowing the plant to adapt to environmental changes by modifying leaf size and anatomy. Moreover, a leaf is made up of various distinct cell layers, each having specialized functions. To understand functional adaptation and the development of the leaf it is essential to obtain cross sections throughout leaf development and at maturity (Kalve et al., 2014). Here, we describe a protocol for transverse sectioning of Arabidopsis thaliana leaves using resin embedding. This protocol provides a reliable platform to yield high quality images of cross sections allowing study of development of various tissue layers across the transversal axis of the leaf. As this method is an adaptation of the protocol developed for the Arabidopsis root tip by Beeckman and Viane (1999) and De Smet et al. (2004), it can easily be modified to accommodate other organs and species.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/148ecc/129698.pdf
Handle