Publication
Title
Transverse sectioning of **Arabidopsis thaliana** leaves using resin embedding
Author
Abstract
The leaf is the major functional part of the shoot performing the bulk of photosynthetic activity. Its development is relatively plastic allowing the plant to adapt to environmental changes by modifying leaf size and anatomy. Moreover, a leaf is made up of various distinct cell layers, each having specialized functions. To understand functional adaptation and the development of the leaf it is essential to obtain cross sections throughout leaf development and at maturity (Kalve et al., 2014). Here, we describe a protocol for transverse sectioning of Arabidopsis thaliana leaves using resin embedding. This protocol provides a reliable platform to yield high quality images of cross sections allowing study of development of various tissue layers across the transversal axis of the leaf. As this method is an adaptation of the protocol developed for the Arabidopsis root tip by Beeckman and Viane (1999) and De Smet et al. (2004), it can easily be modified to accommodate other organs and species.
Language
English
Source (journal)
Bio-protocol
Publication
2015
Volume/pages
5:18(2015), p. 1-5
Article Reference
e1592
Medium
E-only publicatie
Full text (open access)
UAntwerpen
Faculty/Department
Research group
[E?say:metaLocaldata.cgzprojectinf]
Growth and development of higher plants (MARS).
Regulation of cell elongation during Arabidopsis thaliana development.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identification
Creation 19.12.2015
Last edited 03.05.2016
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